Fluorescence and fluorescence microscopy

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the selective localization of cell compounds including antigens, nucleic acid structures or other molecules. Fluorescent techniques are especially useful to localize the intra- and extracellular position of biomarkers in the course of gene expression, during cytoplasmic synthesis and migration.