Cellular localization of lectin affinity in tissue sections of normal human duodenum

K. Wurster, P. Peschke, Wolf D. Kuhlmann


Institut für Pathologie, Städtisches Krankenhaus München-Schwabing, D-8000 München, Germany
Labor für Experimentelle Medizin, Institut für Nuklearmedizin DKFZ, D-6900 Heidelberg, Germany
Peroxidase (HRP) conjugates of Arachis hypogaea agglutinin (PNA), Dolichos biflorus (DBA), Ulex europaeus agglutinin I (UEA I) and Bandeiraea simplicifolia agglutinin I (BSA I) are used to detect the expression of carbohydrate moieties in glycoconjugates of normal human duodenal mucosa. Lectin histology is performed on sections from paraffin embedded specimens of donor blood groups A, B, AB and 0.