Wolf D. Kuhlmann
Division of Radiooncology, Deutsches Krebsforschungszentrum, D-69120 Heidelberg
Laboratory Diagnostics & Cell Science, 56112 Lahnstein, Germany
A range of substrates is available for the cytochemical staining of peroxidase activity. The most utilized substrates are diaminobenzidine for brownish color, aminoethylcarbazole for reddish reactions and chloronaphthol to give a blue-black product. Thus, in double or triple staining experiments, antigens can be subsequently stained in the same tissue section.