Washing solutions

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

Laboratory Diagnostics & Cell Science, 56112 Lahnstein, Germany

Between the various immunohistological incubation steps, cell preparations (sections, cytospins, cell cultures etc.) must be treated by washing solutions in order to take off unreacted substances which might interfere with reagents added in subsequent stages. The type of washing as well as the type of appropriate washing solution will depend on the material under study. We give here a selection of the most applied washing buffers for routine immunohistology.