Cell science

In this section, development paths of microscopy, histology and molecular-specific staining principles are presented. You will find descriptions of the preparation of reagents and their use for biomarker detection. Application examples underline their usefulness for the study of cellular differentiation in ontogenesis, cancer research and histopathology.

Microscopy, histology

Studies on cell structure and biological function are governed by microscopy. The integration of traditional and advanced techniques gives important insights into cellular and molecular details. In this context, molecular probes and biomarkers of high sensitivity and specificity are the key. The methods are complex and not easy at all, especially when multiple biomolecular probes are combined in staining procedures. This chapter and the following ones are intended to spotlight developments in microscopy and histology.

Antibodies, marker molecules, conjugation

In histology, the available range of specific antibodies, molecular probes and well-defined marker molecules enable the study of fine structural details. To this aim, labels such as fluorophores, enzymes or colloidal structures can be used as reporters in taylored assay formats.

Applications in cell science

Application of cell staining methods in the study of animal and human tissues. Alpha-fetoprotein, enzymes and glycoconjugates as biological markers in experimental models and in histopathology.

References

Collection of publications on microscopy, microtechniques and cytochemistry.