In this section, development paths of microscopy, histology and molecular-specific staining principles are presented. You will find descriptions of the preparation of reagents and their use for biomarker detection. Application examples underline their usefulness for the study of cellular differentiation in ontogenesis, cancer research and histopathology.
Studies on cell structure and biological function are governed by microscopy. The integration of traditional and advanced techniques gives important insights into cellular and molecular details. In this context, molecular probes and biomarkers of high sensitivity and specificity are the key. The methods are complex and not easy at all, especially when multiple biomolecular probes are combined in staining procedures. This chapter and the following ones are intended to spotlight developments in microscopy and histology.
Antibodies, marker molecules, conjugation
In histology, the available range of specific antibodies, molecular probes and well-defined marker molecules enable the study of fine structural details. To this aim, labels such as fluorophores, enzymes or colloidal structures can be used as reporters in taylored assay formats.
Selective cell staining
Molecular cell labeling can be performed by either direct or indirect assay principles for which one can select from a large number of detection formats. Generally, staining techniques are dominated by special needs of tissue preparation for the molecules under study.
Here, you can find a collection of frequently used cytochemical and biochemical reagents or solutions in cell research as well as for standard histology. It is reminded that reagent quality has an important impact on cellular staining results.
Applications in cell science
Application of cell staining methods in the study of animal and human tissues. Alpha-fetoprotein, enzymes and glycoconjugates as biological markers in experimental models and in histopathology.